4) Laminar Air Flow Chamber
Laminar
Air Flow Chamber is like a chamber which consists of an air blower in rear side
of the chamber, through the air blower which produces air flow with uniform
velocity along parallel flow lines. Laminar air flow has the specialized filter
system called as High Performance Air Flow (HEPA) which can able to remove
particles as small as 0.3 mm. There is a mechanism in blower, it produces air
velocity along parallel flow lines, and it used to transfer the microbial
culture aseptically. When air blown through the specialized filters, do not
allow to enter the any microbes inside the working space. Procedures like
pouring of media, plating, slant preparations, streaking etc. are performed
without contamination. Inside the LAF a UV tube is fitted which are commonly
used to for surface sterilization of working space before starts the procedure.
It also has the Bunsen burner or spirit lamp to prevent the contamination
during experiment. There are two types of laminar
flow hoods are available, they are vertical or horizontal air flow in the
flasking area. The vertical flow the air moves from the top of the working area
to the bottom whereas, horizontal air flow the air moves from the back of the
working area to the front.
5. Incubator
A device used to grow and maintain
microbiological cultures or cell cultures is known as Incubator, which maintains optimal temperature, humidity, CO2
and oxygen content inside. Incubators are indispensable for culture both
bacterial as well as eukaryotic cells, experimental work in cell biology,
microbiology and molecular biology. Incubator is consisting of double walled
chamber it made up of copper or steel around which warm water or air is
circulated by electric current. It has a thermostat to control the temperature
constantly inside the incubator. The temperature of the incubator chamber can
be adjusted to a desired temperature by means of external knob controlling the
thermostat system. The incubator was fitted with thermometer on the top to
check or monitor the temperature between the gaps of two walls. The main
purpose of using the incubator in microbiological laboratory is growing the
particular microorganism at desired temperature, because temperature acts an
important factor in growth of the microorganism.
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